Growing the highest quality rotifer cultures for feeding larval fish, crustaceans and corals has never been easier than with Easyroti.
Dosage will depend on the number of rotifers being fed. The average consumption is of 4 ml Easyroti per litre of rotifer culture per day (25% of rotifer will be harvested every day).
How to keep a rotifer culture:
- 3 cylinder flasks of 2 litres with slow bubbling.
- Rotifer inoculums.
Culture cycle from strain:
- 5 days.
Recommended sea water density:
- 1019, although rotifers can be cultured well up to 1022.
- 22ºC to 28ºC. The optimum temperature is 24ºC.
To filter the rotifer you should use a 50 micron net. Once you start the culture you will be able to harvest rotifers after 6 days. You will have one flask for the "strain" and another one for the "production" culture.
The information that follows describes the dosage to be used in ideal conditions assuming that the rotifer density is the ideal. Sometimes it is possible that the amounts of mircoalgae that are being added do not adjust to the rotifer demand. This is because in aquariums people don;t count rotifers, so the amount of microalgae added is not exact to the amount of rotifer. If the culture water gets clear this would mean that the amount of microalgae wasn't enough. If rotifer remains with no food for more than 4 hours, the number of eggs will decrease and so will the population growing yield. It is essential that the rotifer always have food.
Rotifers are very sensitive to changes of water parameters. Rotifer population would be affected if the water quality decreases. If you observe an excess of dirtiness in the cylinder you should filtered the rotifer and start the culture in a new clean flask and add the amount of microalgae needed.
Depending on the intensity of the green colour that the flask has, this will indicate the amount of microalgae needed. With time and experience. the difference in green colour will let us do our own feeding protocols. THere is no difference in using live or Easyroti as food.
We will put a glass tube with a gentle bulling (5-10 bubbles per second) in the graduated flask used for the rotifer culture. This air bubbling will provide a homogeneous mixture in the culture. An excessive water movement will make rotifers to loose their eggs and this way they won't hatch.
It is recommended to feed twice a day. But due to the lack of time, rotifers are usually fed once a day.
Initial conditions: "Strain" culture with 500 ml of sea water, number of rotifers 400 rot./ml to 600 rot/ml and 4 ml of easyroti.
Final conditions: The culture will end up with a volume of 1 litre with microalgae.
Instructions: Resuspend 4 ml of Easyroti in 0.5 litres of sea water. Add the rotifer inoculums and add sea water until the total volume is 1 litre.
Initial conditions: 1 litre of rotifer culture. It will be nearly clear and with no microalgae. Estimated rotifer number: 500 rot./ml
Final conditions: The culture will end up with a volume of 1.4 litres with microalgae.
Instructions: Resuspend 4 ml of Easyroti in 0.5 litres of sea water and add 0.4 litres to the rotifer culture.
Initial conditions: 1.4 litres of rotifer culture. It will be nearly clear and with no microalgae. Estimated rotifer number: 500-600 rot./ml.
Final conditions: The culture will end up with a volume of 1.8 litres with microalgae.
Instructions: Resuspend 8 ml of Easyroti in 0.5 litres of sea water and add 0.4 litres to the rotifer culture.
Initial conditions: 1.8 litres of rotifer culture. It will be nearly clear and with no microalgae. Estimated rotifer number: 600 rot./ml.
Final conditions: The culture will end up with a volume of 2 litres with microalgae.
Instructions: Resuspend 6 ml Easyroti in 0.25 litres of sea water and add 0.2 litres to the rotifer culture.
Initial conditions: 2 litres of rotifer culture. It will be nearly clear and with no microalgae. Estimated rotifer number: 700-800 rot./ml.
From this "strain" culture we will get a volume of 0.5 litres to start a new "strain" culture in another 2 litre flask and start a new culture cycle from day 1.
We will add Easyroti to the old "strain" culture, from now on "production" culture, until it reaches 2 litres. If the cylinder is very dirty then we will pour the culture to a clean flask and carry on with the production culture.
Instructions: To the "production" culture we will add 8 ml of Easyroti in 0.5 litres of sea water. For the "strain" culture start a new cycle from day 1.
Initial conditions: "Strain" culture the same as day 2. "Production" culture 2 litres with 800 rot./ml, it will be nearly clear and with no microalgae.
The "production" culture would be used to harvest 0.5 litres every day and be used as food for the aquarium (if you don't feed the aquarium you must do the harvest anyway so you can feed the rotifer with microalgae). If you harvest 0.5 litres of rotifer you always have to add 0.5 litres of sea water with 8 ml of Easyroti.
The "production" culture will be kept until the flask is dirty (5-7 days).
When the "strain" culture reaches day 5 of culture it will be converted to the "production" culture and a new "strain" culture will be created, the same way as it has been explained on day 5.
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